The fold of β-helical proteins contains a repetitive helical strand-loop motif, where each repeat contributes a strand to one or more parallel β-sheet(s). A good example of tubular proteins is the β-helix protein fold. Some naturally occurring proteins contain a tubular or fibrillar motif in their folds. The Protein Data Bank (PDB) is populated by an extensive repertoire of building blocks, with different shapes, sizes, and chemical properties which can be used in rational design of protein-based nanostructures. A building block is a well-defined secondary structural unit which, if cut from the protein chain and placed in solution, is still likely to have a conformation similar to the one it has when embedded in the native protein structure. Finally, we introduce a protocol to design hybrid materials based on the conjunction of functional amyloids and synthetic polymers.Ĭonstruction of stable nanostructures using natural building blocks is a reasonable and promising strategy toward precisely and quantitatively controlling the supramolecular assemblies. Next, we introduce a related method to construct nanostructures based on amyloid peptides. We first introduce a protocol for designing self-assembled nanostructures from naturally occurring protein motifs, followed by structural enhancement by synthetic amino acids. In this chapter we describe a computational and experimental protocol, based on our previous and current work. The use of advanced simulation methods and efficient modeling algorithms, in addition to the rapidly increasing amount of data in DNA, RNA, and protein databases, can considerably accelerate the design process via fast probing of many possible models in a high-throughput cost-effective way, aiming to experimentally test only feasible models. Advances in peptide synthesis and molecular engineering techniques have made self-assembly of peptide segments a favorable route by which to obtain nanostructures, particularly those consisting of single or associated tubes, fibers, and vesicles.Ĭomputational methods have become a powerful tool in nanobiology and nanostructure design. Much work has been done in recent years in the design and construction of nanostructures using DNA, RNA, and protein segments. Exploiting the natural ability of macromolecules to self-assemble can be a very useful approach in the design and construction of novel molecular structures. In recent years there has been much focus on the experimental and computational design of self-assembled nanomaterials based on the self-assembly properties of proteins. ![]() The shapes, sizes, and functions of these structures are determined by the amino acid sequence of these proteins. These molecules may create large complexes of well-defined structures and functions. In nature, protein domains often self-assemble, spontaneously organizing in stable higher-order structures through noncovalent interactions. Nanotechnology applications include targeted drug delivery systems, computational devices, and scaffolding tissues. Nanotechnology aims to design novel materials and molecular devices, often by exploiting the natural ability of molecules to self-assemble into larger, ordered structures at the nanoscale. We focus on the principles of nanostructure design with naturally occurring proteins and synthetic amino acids, as well as hybrid materials made of amyloids and synthetic polymers. ![]() Herein, we summarize a protocol for designing nanostructures consisting of self-assembling building blocks, based on our recent works. The introduction of engineered synthetic residues or short peptides into these building blocks can greatly expand the available chemical space and enhance the desired properties. Structural databases contain large libraries of protein molecules and their building blocks with a range of sizes, shapes, surfaces, and chemical properties. Autonomous biological building blocks with available 3D structures provide an extremely rich and useful resource. The design includes functional synthetic materials and biological macromolecules. ![]() Nanodesign requires the ability to predictably manipulate the properties of the self-assembly of autonomous building blocks, which can fold or aggregate into preferred conformational states. In recent years there has been increasing interest in nanostructure design based on the self-assembly properties of proteins and polymers.
0 Comments
Leave a Reply. |
Details
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |